LPS-induced up-regulation of B7.2/CD86, but not CD23, is impaired in MD-1–deficient B cells.

Purified B cells from wild-type (upper panels) or MD-1–deficient (lower panels) mice were stimulated with lipid A (1.0 μg/mL), anti-RP105 (10 μg/mL), or anti-IgM (10 μg/mL), as indicated. After 1 day, cells were harvested and stained with anti-B7.2 (A) or anti-CD23 (B). Open histograms (A) and gray lines (B) show cells stimulated with medium alone. Similar results were obtained from 3 independent experiments.