Cytogenetic and molecular genetic characterization of TI-1 cell line.

G-banding (A) and SKY in classification colors (B) demonstrate several numerical and structural aberrations. Arrows indicate clonal structural abnormalities. See text for complete description. The asterisk in A designates der(6)t(3;6)(q2?5;p2?5), an abnormality present in a minority of TI-1 cells. (C) CGH profile shows regions of gain and loss in TI-1. Upper and lower threshold limits are 3.0 standard deviations from the mean signal intensity. Note the high-level amplification of 22q11. (D) Portion of RLGS profile of normal blood (control) on top and TI-1 on bottom. RLGS locus 3C71 (arrow) on 22q11 is amplified in TI-1, as demonstrated by the increased signal intensity compared to the control. (E) Dual color FISH with probe for BCR on 22q11 (green signals on 2 normal chromosomes 22) and ABL on 9q34 [red signals; 1 signal located on del(9)(p13p24) and 2 signals on both ends of der(9)t(9;9)(p1?3;q22)] shows multiple, overlappingBCR/ABL fusion signals (yellow) localized on 2 der(22)t(9;13;22) (arrows) and 1 der(2)t(2;9;22) (arrowhead).