Expansion of CFU-GEMM in the AGM/FL coculture.

Immature hematopoietic progenitors in floating cells generated in vitro were examined by colony assays. GFP⁺ cells were sorted from total hematopoietic cells generated and used for an in vitro colony-formation assay. As a control, CD34⁺/c-Kit⁺ cells in freshly isolated E11.5 AGM were similarly tested. Fold expansion relative to the control level is shown in the figure. The number and type of colonies were determined at day 10. Data shown are mean values of at least triplicate experiments.