Proliferation of LCMV-specific T cells.

Groups of CCR5^−/− and wild-type (wt) mice were infected with 10³ LD₅₀ of LCMV Traub and given BrdU in their drinking water for 3 days prior to postinfection days 8 (day 5-8), 10 (day 7-10), or 14 (day 11-14). Splenocytes were harvested and the cells were stained with anti-CD8 Cy or anti-CD4 Cy and anti-IFN-γ PE and anti-BrdU FITC. The mice were evaluated by flow cytometry for the presence of (A) CD8⁺ BrdU⁺IFN-γ⁺ T cells or (B) CD4⁺ BrdU⁺IFN-γ⁺ T cells. Representative dot plots from 1 of 2 experiments are presented. Three mice per group were included in each experiment.