Fig. 7.
Fig. 7. Effect of PI3 kinase inhibition on expression of cell cycle regulatory proteins. / Parental 32D or 32D-TEL/PDGFβR cells were collected in log phase growth (lanes 1, 8) or replated in the presence of DMSO or LY294002 at 25 μM for the indicated period of time. Cells were lysed in 1% Triton lysis buffer; protein quantitated, separated by SDS-PAGE, and blotted. Lysates were probed with antibodies for the indicated proteins.

Effect of PI3 kinase inhibition on expression of cell cycle regulatory proteins.

Parental 32D or 32D-TEL/PDGFβR cells were collected in log phase growth (lanes 1, 8) or replated in the presence of DMSO or LY294002 at 25 μM for the indicated period of time. Cells were lysed in 1% Triton lysis buffer; protein quantitated, separated by SDS-PAGE, and blotted. Lysates were probed with antibodies for the indicated proteins.

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