mRNA expression levels of ODC and type XVIII collagen.

Total RNA was extracted from breast cancer tissues and the noncancerous adjacent tissue. (A) RT-PCR was performed by using primers specific for ODC. Band intensities were quantitated by using National Institutes of Health image software and normalized to the intensity of the GAPDH signal. Straight and broken lines indicate normal mean and mean + SD levels, respectively. (B) ODC overexpressing breast cancer tissue (15 pairs) was analyzed for type XVIII collagen mRNA expression. Band intensities were quantitated by using National Institutes of Health image software and normalized to the intensity of the GAPDH signal. The line indicates the normal mean level.