PCR-SSCP analysis of the C/EBPα gene locus.

(A) Abnormally migrating bands in lane 3 of both panels for the MDS (RAEB-t) patient F3881. Region 2 is a 37-bp duplication and region 4 is a 1015G>T. The other lanes are derived from genomic DNA samples of other MDS patients and display bands with normal mobility. (B) Abnormally migrating bands in lane 3 of both panels for the AML-M2 patient F3820. Region 1 is a 1-bp deletion of C381, and region 4 is a 24-bp duplication of nt 1062 through 1085. The other lanes are derived from genomic DNA samples of other AML patients and display bands with normal mobility. (C) Abnormally migrating bands for region 4 are detected in lanes 1 (patient F3901), 10 (patient J2), 11 (patient J3), and 14 (patient J6). Lane 1 is a 3-bp deletion of nt 1083 through 1085; lane 10 is a transversion of 1001C>A; lane 11 is 15-bp duplication of nt 1083 through 1097; and lane 14 is a 3-bp duplication of nt 1083 through 1085. The other lanes are derived from genomic DNA samples of other AML patients and display bands of normal migration. The arrows at the left and right of each panel indicate the positions of the shifted bands.