Predicted amino acid sequence comparison of AML region-4 mutations with WT C/EBPα and the family members C/EBPβ, C/EBPδ, and C/EBPε.

The predicted amino acid sequences from residues 272 through 354 for the AML region-4 mutants were aligned with that of WT human C/EBPα, C/EBPβ, C/EBPδ, and C/EBPε. The consensus sequence was derived by Vinson et al1 for 11 bZIP family members. The consensus basic (B) residues and leucine residues in each amino acid sequence are highlighted in bold text above the consensus sequence. The first conserved leucine is a threonine for C/EBPs. The duplicated amino acid residues for samples F3820, J3, and J6 are indicated by bold text. The Arg305Pro change in sample K-6 is indicated by the shaded box. The basic, fork, and leucine zipper regions are indicated above and below the alignments. The fork region is an invariant 7–amino acid residue spacer region between the first leucine in the zipper and the conserved basic region.1 The alignments were performed by means of AlignX (Vector NTI Suite v.6.0; Informax, Bethesda, MD).