Locus of WT and mutant C/EBPα.

WT and mutant forms of C/EBPα localize in the nucleus. NIH3T3 cells were transfected with the MIG retrovirus constructs expressing the mutant samples F3901, J3, and F3820. The negative control cells were transfected with empty MIG, and the positive control cells were cotransfected with pCMV-C/EBPα₄₂ and pEGFPc. The MIG retrovirus constructs express GFP along with the gene of interest, thereby allowing identification of transfected cells. All GFP⁺ cells, except for the empty vector control (MIG), showed nuclear staining for wildtype or mutant C/EBPα whereas all GFP⁻ cells were negative for C/EBPα expression.