Fig. 1.
Effect of tax on expression of CCR4 and CCR7 mRNA.
Total RNA was prepared from PBMCs stimulated with phytohemaggutinin (PHA) for 3 days and JPX-9 treated without (control) or with 10 μM of Cd2+ for 3 and 7 days. RT-PCR was carried out for CCR4, CCR7, IL-2 receptor α chain (IL-2Rα), Tax, and G3PDH as described in “Materials and methods.” (1) No RNA. (2) PHA-stimulated PBMCs (positive control). (3) Untreated JPX-9. (4) JPX-9 treated with Cd2+ for 3 days. (5) JPX-9 treated with Cd2+ for 7 days.