Surface expression of CD8 antigen on NK cell clones.

NK cell clones were incubated with mAb recognizing CD8 (Leu2a) or KIR2D (NKVFS1) or CD94/NKG2 complex (HP-3B1), followed by PE-conjugated GAM, and analyzed on a FACSort. Results are expressed as Log red fluorescence intensity (au) versus number of cells. Left histograms in each panel represent fluorescence of samples incubated with an unrelated mAb followed by GAM-PE. Results are representative of 90 clones isolated from 3 different healthy donors.