Fig. 3.
Impaired macrophage proliferative responses by CP.
Alveolar macrophages were obtained from PBS-treated control mice or from mice treated with CP (days 4, 10, and 14 after the first CP). Macrophages were cultured without or with stimulation by GM-CSF (4 ng/mL) for 3 days and were pulsed with 3H-thymidine. Cell proliferation rate is expressed as counts per minute by beta-counting. Results are expressed as mean ± SEM from 2 independent experiments (replicate wells per condition in each experiment). The difference between the control and mice at days 4 and 10 is statistically significant.