Expanded alveolar macrophage population by GM-CSF gene transfer.

(A) Mice received intranasally a dose of control gene transfer vector (Addl70.3) or GM-CSF gene transfer vector (AdGM-CSF) at day 5 after the first CP and received 2 additional doses of CP and were then killed at day 10 after the first CP (day 1 after the last CP). (B) Mice received Addl70.3 or AdGM-CSF gene transfer vector either intranasally or intramuscularly at day 10 after the first CP (1 day after the last CP) and were killed at day 15 after the first CP. The number of alveolar macrophages was determined on cytospins. Results are expressed as mean ± SEM from 4 mice (CP/AdGM-CSF) for panel A. The results (intranasal delivery) presented in panel B are expressed as mean ± SEM from 3 mice per group and are representative of 3 independent experiments.