Expression of SOCS-2 and related SOCS proteins after short-term exposure to STI571.

(A) Northern blot of KYO1 cells exposed to 1 μM STI571 for the indicated lengths of time. (B) Real-time RT-PCR from KYO1 cells exposed to 1 μM STI571 for various lengths of time. Results are expressed as ratios SOCS-2/GAPDH as compared with cells not exposed to STI571. (C) Immunoblot from BV173 cells exposed to 1 μM STI571 for the indicated time. Numbers below the blots represent the densitometric ratios SOCS-2/actin as compared with the nonexposed cells. (D) Northern blot of KYO1 cells exposed (+) or not (−) to 1 μM STI571 for 10 hours, probed with SOCS-1, SOCS-3, and CIS.