SOCS-2 inhibits colony formation and decreases the adhesive capacity of 32Dp210 cells.
(A) Cells were plated in triplicate in methylcellulose with or without 1 μM STI571, and numbers of granulocyte macrophage–colony-forming unit (CFU-GM) colonies were determined after 7 days of culture. Results represent the mean plus or minus standard deviation of colony numbers, normalized to 103 plated cells. (B) Cells were plated in quadruplicate in 96-well flat bottom plates. After 24 hours and 2 washes in PBS, numbers of adherent cells were determined by MTS staining. Data are expressed as a ratio adherent–total number of cells. (C) Cells were plated in a 48-well tissue culture plate coated with the CH-296 fibronectin fragment. The number of migrating cells was assessed as described.26 Results were normalized against the cells kept in wells under the same conditions with PBS/2% BSA. (D) Cells were plated in the top chamber of a 5.0-μm Transwell plate. After 24 hours, the numbers of cells that had migrated to the lower chamber (dark bars) and those that remained in the Transwell (light bars) were determined by hemocytometer counting of trypan blue–stained cells.
