Phenotypical analysis of human thymocytes recovered from NOD/SCID mice.

Conditioning indicates radiotherapy + TM-β1. Unless otherwise indicated, gates were set on viable human CD45⁺ cells (A, B, C, E, H, I). Within this population subsequent maturational stages were identified: CD4⁺CD8⁺ (68%), CD3⁺CD69⁺ (13%), CD3⁺CD27⁺ (7%), and CD3⁺CD1⁻ (2%). Virtually all CD3⁺cells were TCR-αβ⁺. (D) Gated on CD45⁺CD3⁺ cells. (F, G) Gated on CD3⁺CD1⁻ cells, as indicated.