American Society of Hematology

Fig. 4.

$Fig. 4. Vβ-Cβ RT-PCR heteroduplex analysis of thymus and bone marrow. / RNA was isolated from thymus (sorted CD45+CD3+CD27+CD69+fraction) (A) and bone marrow (total, unsorted) (B), and after reverse transcription cDNA was amplified with PCR. On heteroduplex analysis of the thymus, polyclonal smears can be observed for most Vβ families. Bone marrow shows a pauciclonal pattern superimposed on polyclonal background smears.$

Vβ-Cβ RT-PCR heteroduplex analysis of thymus and bone marrow.

RNA was isolated from thymus (sorted CD45⁺CD3⁺CD27⁺CD69⁺fraction) (A) and bone marrow (total, unsorted) (B), and after reverse transcription cDNA was amplified with PCR. On heteroduplex analysis of the thymus, polyclonal smears can be observed for most Vβ families. Bone marrow shows a pauciclonal pattern superimposed on polyclonal background smears.

This Feature Is Available To Subscribers Only