TREC analysis on human thymocytes and peripheral T cells harvested from mice injected with purified CD34⁺ cells.

Real-time PCR quantification of TRECs was performed on human CD3⁺ cells harvested from several organs of mice injected with purified CD34⁺ UCB cells 3 months earlier. CD3⁺CD45RA^low and CD45⁺CD3⁺CD45RA⁺ cells sorted from spleen and mesenteric lymph nodes (MesLN) and CD45⁺CD3⁺CD27/69⁺ cells sorted from the thymus.