Extracellular ATP induces CCR7 and CXCR4 and down-regulates CCR5 mRNA.

DCs were generated from purified peripheral blood CD14⁺monocytes by culture with granulocyte-macrophage colony-stimulating factor and IL-4. On day 6, CD2⁺ and CD19⁺ cells were removed; resulting cells were more than 97% CD1a⁺ and CD14⁻. Immature DCs were not treated (nt), stimulated with 250 μM ATP or UTP, or induced to mature with 10 μg/mL LPS in the absence or presence of nucleotides. After 16 hours, total RNA was extracted and RNase protection assay done to visualize chemokine-receptor mRNA transcripts. Shown is one representative experiment of 3 done.