ATP up-regulates CXCR4, CCR7, and CD83 and reduces CCR5 on DC membranes.

Immature DCs were untreated, stimulated with 250 μM ATP or UTP, or induced to mature with 10 μg/mL LPS in the absence or presence of nucleotides. After 24 hours, DCs were stained with mAbs against the indicated surface molecules (bold histograms) or with isotype-matched control Ig (thin histograms). Numbers indicate the net mean MFI. Results are representative of 5 independent experiments.