Deletion and mutational analysis of the cis-elements required for HTLV-I Tax-induced MMP-9 promoter activity.

Arrow indicates transcription start site, and × indicates the sites of mutation in the MMP-9 5′ flanking sequence inserted upstream of the CAT gene. Open and solid bars represent CAT activity of pHβAPr-1-neo (−Tax) and β-actin–Tax (+Tax)–transfected Jurkat cells, respectively. The activities are given relative to the activity of cells transfected with the empty vector (pHβAPr-1-neo) and −73 CAT, which was defined as 1. Data are also expressed as fold inductions in CAT activity in Tax-transfected cells over that in empty vector-transfected cells. The mean values and SD bars represent the results of 3 experiments.