Binding of nuclear proteins from HTLV-I–infected T cells to the NF-κB and SP-1 probes derived from the MMP-9 promoter.
Nuclear extracts were prepared from HTLV-I–infected (lanes 4-8) and uninfected T cells (lanes 1-3) and incubated with either NF-κB (A) or SP-1 32P-labeled probes (B). Competition assays were performed with 100-fold excess amounts of each specific competitor oligonucleotide in nuclear extracts from HPB-ATL-2 cells (A, lanes 14-17; B, lanes 9-12). Supershift assay of NF-κB DNA-binding complexes in HPB-ATL-2 cells was also performed. Where indicated, appropriate antibodies were added to the reaction mixture before addition of 32P-labeled probes (A, lanes 9-13). Arrowheads show the DNA-binding complexes supershifted by antibodies.
