Fig. 4.
![Fig. 4. Immunoprecipitation analysis of chymotrypsin-treated platelets. / Paternal platelets (Oea [+], HPA-1a/1b, HPA-4a/4a) were surface labeled with biotin, digested with chymotrypsin, lysed, and precipitated with anti–HPA-1a (lane 1), anti-Oea (lane 2), anti-HPA-4a (lane 3), and normal human serum (lane 4). Immunoprecipitates were analyzed on 7.5% SDS-PAGE under nonreducing conditions, blotted, and visualized with chemiluminescence substrate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/4/10.1182_blood.v99.4.1205/6/h80422165004.jpeg?Expires=1735822773&Signature=2~M0RCn6-UFIHuxucE6Nrja4JJWtvEHM9qoPcxOb~vA8GWIlHBn86iM18Yr8dtJXIehWLzuFTOPECNas-TpQG1ivu2O0bHY5Mjq2CeaKXXo0CQ2CWwnZGrg~c~xEBSnOEqhbImkBpnItoaX1~K38uATOzrQOYWLzjJXvQWfUH~sLZIZRbdh4E9DIe5U1imOaFkGSqsIHgpgZrQyJbLzKXHpR49wPVQv8wcpVSebIzwoqTbZNVBcxtYRPS7-WAzfk1S-C3OplNRtmqBRJi8dmp-m6Pbu3FRYDZHss9A9cP~iy7v6OoPIohts-~054VGgwts1R96py2DKnRy-gXJ5q6A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation analysis of chymotrypsin-treated platelets.
Paternal platelets (Oea [+], HPA-1a/1b, HPA-4a/4a) were surface labeled with biotin, digested with chymotrypsin, lysed, and precipitated with anti–HPA-1a (lane 1), anti-Oea (lane 2), anti-HPA-4a (lane 3), and normal human serum (lane 4). Immunoprecipitates were analyzed on 7.5% SDS-PAGE under nonreducing conditions, blotted, and visualized with chemiluminescence substrate.
