Location of ΔLys611 in GPIIIa cDNA and genomic DNA.

(A) Location of the AAG deletion in exon 10. The arrow indicates the cleavage site of DraI endonuclease in cDNA from Oe^a (+) individual. The recognition site of the enzyme is underlined. (B) Genotyping analysis of Oe^a alloantigen by PCR-SSP. The genotyping of Oe^a-phenotyped individuals (father and grandfather) using wild-type primer (lanes 2 and 4) or mutant primer (lanes 3 and 5) is shown. The 439-bp fragment represents the internal control. Note that reverse primer (arrow) specific for mutant (T) and wild-type allele (G) amplified products with 3 bases different in length (200 bp versus 197 bp).