Overexpression of Gab2 activates the c-fos SRE transcriptional activity in K562 cells.

K562 cells (2 × 10⁶) cells were transfected with a total of 4.7 μg DNA consisting of 0.5 μg c-fos SRE luciferase reporter (pSRE-luc), 0.2 μg pCMV-βgal internal control vector, and 2 μg of each test construct. Where a single test construct was used, pcDNA3.1 was added to keep the amount of total DNA constant. At 48 hours after transfection, luciferase activity was measured and normalized to β-galactosidase activity. Data shown are the means and SDs of 3 or more independent experiments performed in duplicate.