Fig. 3.
![Fig. 3. C2-ceramide inhibitition of PLD activity in PMNs stimulated for superoxide production and degranulation. / PMNs were labeled with 1-O-[3H]-octadecyl-sn-glycero-3 phosphocholine. PMNs were treated with lipids as in Figure 1, then incubated with 1% ethanol for 5 minutes at 37°C. Cells were then stimulated at 37°C with G-CSF (50 ng/mL, 10 minutes) (panel A) or cytochalasin B (5 μg/mL, 3 minutes) (panel B) followed by FMLP (1 μM, 5 minutes). Cell pellets were extracted with chloroform and methanol. Lipid products were separated by means of TLC, and [3H]-labeled PEt was removed and counted in a scintillation counter. Data represent the mean ± SEM of 4 experiments. *Significantly different from control;P < .05.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/4/10.1182_blood.v99.4.1434/6/h80422119003.jpeg?Expires=1735570602&Signature=OReiHRG3CJes6ILpn0n9Q~yXR6Hju8At8sUBGa~MJ44FjpAjuJzmAGtlt3Kn2ZZRXb8oJWFvAo~PrFk6R178EKJnrB7IMz1oFKpStnsCB-0YQCfZTHQ0fJlL072wNRDSYYr7JLzl7pIGCujlMTxp707zZDhiVdJCKZ~ViWwKDolsqGXtiDMo73REFIIJjKTQpw~7El3AlHi5vVzjvooms0Xg5rAsdDys2mxCUr745N1OB1cKoHuofzVC4BR9j3RU60L3PGK0qwJFNhVGmN5YW9zjY~Em81PWWI6LZcfoW-4bBYI4eZmTiU-MyEXX8Hw5SJLOO3eFiN4VyCiWa0BgVg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
C2-ceramide inhibitition of PLD activity in PMNs stimulated for superoxide production and degranulation.
PMNs were labeled with 1-O-[3H]-octadecyl-sn-glycero-3 phosphocholine. PMNs were treated with lipids as in Figure 1, then incubated with 1% ethanol for 5 minutes at 37°C. Cells were then stimulated at 37°C with G-CSF (50 ng/mL, 10 minutes) (panel A) or cytochalasin B (5 μg/mL, 3 minutes) (panel B) followed by FMLP (1 μM, 5 minutes). Cell pellets were extracted with chloroform and methanol. Lipid products were separated by means of TLC, and [3H]-labeled PEt was removed and counted in a scintillation counter. Data represent the mean ± SEM of 4 experiments. *Significantly different from control;P < .05.
