PAC/BAC contig covering the

CHIC2 locus at 4q11-q12 and localization of the different breakpoints of the t(4;12)(q11;p13). We previously reported the generation of a PAC contig (PACs 200D9, 238H24, and 1146G14) spanning the CHIC2 locus at 4q11-q12.23 Database searches identified several partially sequenced BAC clones containing the CHIC2 andGSH2 genes, which were aligned to the PAC contig (drawing not to scale). Using PCR and database searches, GSH2 and the 2 exons of HSG2 were located on this contig. The localization of the breakpoints of 6 t(4;12) cases is indicated by an arrowhead and numbered according to the cases as described in the text. Exons are represented by open or filled squares, and the transcriptional orientation is indicated by an arrow. The sequenced BAC clones are represented by their accession numbers. Tel indicates telomeric side; cen, centromeric side; open circle, negative by hybridization or PCR; filled square, positive by hybridization or PCR; x, positive by electronic PCR/database searches.