PAC/BAC/cosmid contig covering the

ACS2 andIL-3/CSF2 loci at 5q31 and localization of the different breakpoints of the t(5;12)(q31;p13).Schematic illustration of the small PAC/cosmid contig linkingACS2 with IL-3/CSF2 (not to scale). Database searches identified several partially or completely sequenced genomic clones containing the ACS2, IL-3, CSF2, and P4HA2 genes, confirming the PAC contig. These clones are represented by their accession numbers. Yagasaki et al22 mapped IRF1 telomeric to the breakpoints of the t(5;12). A 1 Mb contig was previously described33that links ACS2 to IL-5 and is in agreement with the map shown here. The 3 t(5;12)(q31;p13) breakpoints described by Yagasaki et al22 are numbered as Y1, Y2, and Y3. The breakpoint described in this work is indicated with case 7, as described in the text. Genes are represented by boxes, and their transcriptional orientation is indicated by an arrow. The sequenced BAC clones are represented by their accession numbers. tel indicates telomeric side; cen, centromeric side; open circle, negative by hybridization or PCR; filled square, positive by hybridization or PCR; x, positive by electronic PCR/database searches.