Phenotypically mature FL DCs retain impaired allostimulatory activity.
DCs from FL or control mice were cultured overnight as in Figure 4 and used in MLR as in Figure 3. (A) Five thousand DCs isolated from B6D2F1 mice were cultured with 105 T cells isolated from either B6 (allogeneic) or B6D2F1 (syngeneic) mice. T-cell proliferation was determined by 3H-thymidine incorporation. Allogeneic T-cell responses to control DCs (open circle) and to FL DCs (closed circle) and syngeneic T-cell responses to control DCs (open square) and to FL DCs (closed square) are shown. Data are the mean ± SD of quadruplicate cultures. Supernatants were collected at 24 hours for IL-2 (B) and at 72 hours for IFN-γ (C) and were measured by ELISA, as described in “Materials and methods.”
