Angiogenesis in Matrigel assay.

Angiogenesis was measured in Matrigel plugs injected subcutaneously in CD1 mice. Matrigel was added with bFGF (150 ng/mL), PDGF-BB (150 ng/mL), or both. The bFGF-induced vessel formation was significantly reduced in the presence of PDGF-BB. Data were collected on 8 animals for bFGF alone, 6 animals for PDGF-BB alone, 11 animals for bFGF/PDGF-BB treatment, and 8 animals for negative controls. Data are expressed as average ± SD.