Percentage of recovery of total, CD34⁺, and CD34⁻ cells from the BM and spleen of NOD/SCID recipients 40 hours AT.

BM cells partially immunomagnetically enriched for CD34⁺cells (to prepare grafts containing approximately 50% CD34⁺ cells) were stained with CFSE and transplanted into conditioned NOD/SCID recipients. Percentage of CD34⁺ cells was determined in the graft and among CFSE⁺ cells recovered from the BM (light bars) and spleens (dark bars) of recipient mice 40 hours AT. These percentages, the number of cells in the graft, and the number of cells recovered from each tissue were used to calculate the percentage of recovery of total, CD34⁺, and CD34⁻ cells. Each bar represents the mean ± SD of 5 to 7 independent measurements in 6 experiments. No statistically significant differences were detected in any comparison between all 3 measurements from each tissue.