Release of HBP, HSA, and elastase from purified neutrophils.

Neutrophils were stimulated with fMLP (100 nM), cytochalasin B (10 μM), PMA (100 nM), and ionomycin (0.5 μM) for 30 minutes at 37°C. Cells were incubated in the absence of a stimulus for 10, 20, and 30 minutes at 37°C. Cells were pelleted, and the concentrations of HBP (A), HSA (B), and elastase (C) in the culture medium were determined by ELISA. Results are the mean ± SD of 3 independently performed experiments, each performed in duplicate.