Fig. 5. Subcellular localization of HBP by electron microscopy. / Neutrophils were fixed and prepared for transmission electron microscopy as described in “Materials and methods.” (A) Neutrophils were immunostained with a monoclonal antibody against ALP and a rabbit polyclonal antibody against HBP. Bound antibody was detected with gold-labeled secondary antibodies against mouse IgG (6-nm gold particles) and rabbit IgG (10-nm gold particles), respectively. Arrows indicate HBP, arrowheads indicate ALP. (inset) Secretory vesicle containing HBP at a higher magnification. (B) Neutrophils were immunostained with a monoclonal antibody against MPO and a polyclonal antibody against HBP, as described above. Arrows indicate HBP, arrowheads indicate MPO. (inset) Azurophil granule containing HBP at a higher magnification. Images are representative of 4 separate experiments. Bar = 200 nm.
Fig. 5.

Subcellular localization of HBP by electron microscopy.

Neutrophils were fixed and prepared for transmission electron microscopy as described in “Materials and methods.” (A) Neutrophils were immunostained with a monoclonal antibody against ALP and a rabbit polyclonal antibody against HBP. Bound antibody was detected with gold-labeled secondary antibodies against mouse IgG (6-nm gold particles) and rabbit IgG (10-nm gold particles), respectively. Arrows indicate HBP, arrowheads indicate ALP. (inset) Secretory vesicle containing HBP at a higher magnification. (B) Neutrophils were immunostained with a monoclonal antibody against MPO and a polyclonal antibody against HBP, as described above. Arrows indicate HBP, arrowheads indicate MPO. (inset) Azurophil granule containing HBP at a higher magnification. Images are representative of 4 separate experiments. Bar = 200 nm.

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