Fig. 6.
Subcellular localization of elastase and HBP in neutrophils.
Unstimulated (A, C) and fMLP (100 nM)-stimulated (B, D) neutrophils were fractionated as described in “Materials and methods.” Each fraction was analyzed for elastase (A, B) and HBP (C, D) by ELISA. Mean ± SD of 3 independent experiments is shown. (inset) Absolute amounts of HBP in one representative experiment with (♦) and without (■) stimulation of cells.