Fig. 5.
Proliferative responses of splenocytes from wild-type and tyk2−/− mice.
Splenocytes from wild-type and tyk2-deficient mice were activated with anti-CD3 for 48 hours. Viable cells were washed, replated, and incubated in the absence or the presence of various doses of IL-12 (A), IL-18 (B), or IL-18 in the presence of 1 ng/mL IL-12 (C). Cells were pulsed with 3H-thymidine for the last 18 hours of a 48-hour assay.
