Fig. 6.
Changes in lung level of iNOS mRNA in rats administered ET.
Expression of iNOS mRNA was examined in the lungs at the indicated time points after ET (5 mg/kg) administration. Chemiluminograms of typical expression of iNOS mRNA (4.5 kilobases [kb]) from 4 determinations (A) and ethidium bromide staining of 18S ribosomal RNA (1 μg of total RNA per each lane) (B) at each time point are shown. (C) The chemiluminograms of iNOS mRNA expression were quantified, and the results are presented as means ± SD of 4 animals. Pre indicates the time just before ET administration. Mean level of maximal value is arbitrarily set at 100. *P < .01 versus pre.