Effect of AT on increase in the lung level of TNF-α mRNA 60 minutes after ET administration in rats.

AT (250 U/kg) was administered intravenously 30 minutes prior to ET (5 mg/kg) administration. Control animals were administered saline instead of ET. Expression of TNF-α mRNA in the lungs was determined 60 minutes after ET administration. (A) Chemiluminograms of typical expression of TNF-α mRNA (1.6 kb) 60 minutes after ET administration; (B) ethidium bromide staining of 28S ribosomal RNAs (3 μg of total RNA per each lane). (C) The chemiluminograms were quantified by comparison with the mean value seen in the ET plus saline group, arbitrarily set at 100. Data are expressed as means ± SD of 4 animals. *P < .01 versus control. †P < .05 versus ET plus saline.