Effect of anti-rat TNF-α Ab, AT pretreated with indomethacin, or iloprost on changes in MAP in rats administered ET.

Changes in MAP after the administration of ET (5 mg/kg) were determined. (A) Anti-rat TNF-α Ab (0.25 mg/kg, open circles) was administered intraperitoneally 30 minutes prior to ET administration. Control rats were injected with saline plus ET (closed circles). (B) Indomethacin (5 mg/kg) was administered subcutaneously 30 minutes before the administration of AT (250 U/kg), which was administered 30 minutes prior to the administration of ET (open circles). Control rats were injected with saline plus ET (closed circles). (C) Iloprost (100 ng/kg/min, open circles) or saline (closed circles) was continuously injected from 30 minutes before the administration of ET until the end of the experiment. Pre indicates the time just before ET administration. Data are expressed as means ± SD of 5 animals. *P < .01 versus ET plus saline.