American Society of Hematology

Fig. 1.

$Fig. 1. Chemokine receptor expression by tonsillar B-cell subsets. / (A) Expression of the CCR6, CCR7, CXCR4, and CXCR5 transcripts in virgin (V), GC, and memory (M) B cells was analyzed by semiquantitative RT-PCR. (B) Surface expression of CCR6, CCR7, CXCR4, and CXCR5 was performed by triple immunofluorescence analysis of unfractionated tonsillar B cells using a combination of anti-IgD, anti-CD38, and the corresponding antichemokine receptor Abs. (C) Intracytoplasmic expression of CCR6 and CCR7 in virgin, GC, and memory B cells. (D) Surface expression of CCR7 on B cells after a 6-hour culture in complete medium. The data shown are representative of 4 independent experiments. The red lines correspond to the staining with the antichemokine receptor Ab, the black lines to the staining with an irrelevant isotype-matched Ab.$

Chemokine receptor expression by tonsillar B-cell subsets.

(A) Expression of the CCR6, CCR7, CXCR4, and CXCR5 transcripts in virgin (V), GC, and memory (M) B cells was analyzed by semiquantitative RT-PCR. (B) Surface expression of CCR6, CCR7, CXCR4, and CXCR5 was performed by triple immunofluorescence analysis of unfractionated tonsillar B cells using a combination of anti-IgD, anti-CD38, and the corresponding antichemokine receptor Abs. (C) Intracytoplasmic expression of CCR6 and CCR7 in virgin, GC, and memory B cells. (D) Surface expression of CCR7 on B cells after a 6-hour culture in complete medium. The data shown are representative of 4 independent experiments. The red lines correspond to the staining with the antichemokine receptor Ab, the black lines to the staining with an irrelevant isotype-matched Ab.

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