CCR6 and CCR7 expression on memory B cells after 6-hour preculture in complete medium.

Memory B cells were double stained with anti-CCR6 and anti-CCR7 mAbs. The CCR7 expression by CCR6⁺ and CCR6⁻ memory B cells is shown as histograms. The horizontal line indicates the negative threshold as determined by staining with an irrelevant isotype-matched Ab. The data shown are representative of 3 independent experiments.