Chemotactic response of virgin and memory B cells to lymphoid chemokines.

CD38⁻ B cells were precultured for 6 hours in complete medium before being assessed for chemotaxis in response to MIP-3α, MIP-3β, SDF-1 α, and BCA-1 in the Transwell migration assay. Input and migrated B cells were stained with an anti-IgD Ab to calculate the percentage of virgin and memory B cells that migrated during the assay. The results are expressed as percent of input cells specifically migrating in response to the chemokines after subtraction of the percent of cells spontaneously migrating to complete medium containing no chemokine. The chemotactic response of virgin B cells and memory B cells is shown as open and solid diamonds, respectively. Results are expressed as mean ± SD of experimental duplicates. Representative of 3 independent experiments.