Genotype of Donor A by PCR-SSP.

Genomic DNA samples from Donor A, his mother (Donor C), and his son (Donor B) were HPA-1 genotyped by PCR-SSP. The 3 control samples (HPA-1a1a, 1a1b, and 1b1b) show the expected amplicons of 90 bp for HPA-1 (filled arrows) and control amplicons obtained with primers specific for human growth hormone (429 bp; open arrows). The PCR-SSP genotypes of the test samples are HPA-1a1b, HPA-1a1a, and HPA-1a1b for Donor A, Donor C, and Donor B, respectively.