Reactivity of platelets from Donor A with monoclonal anti-β3Leu33 in flow cytometry.

(A) Washed platelets from Donor A and control donors were stained with 3 human β3Leu33-specific mAbs (CAMTRAN-007, 19-7, and 25-13) in a platelet immunofluorescence test. All 3 mAbs show significantly reduced binding to the platelets from Donor A compared to the heterozygous control. (B) Whole blood β3Leu33 phenotyping was performed using FITC-labeled CAMTRAN-007 as described. Reactivity for Donor A is reduced compared to the β3Leu33 homozygous and heterozygous controls. Reactivity for Donor C is reduced to the level observed with a heterozygote. Median fluorescence intensity (MFI) is presented in each case.