Fig. 7.
Fig. 7. The Arg93Gln mutation does impair the β3Leu33-specific mAb CAMTRAN-007 binding to β3 integrins expressed in CHO cells. / Adherent CHO cells were detached with EDTA buffer, washed, and directly labeled with the anti-CD61–PE or CAMTRAN-007–FITC for 30 minutes on ice. Cells were washed and analyzed by flow cytometry. To control for the variations in β3 expression between the different cell clones, CAMTRAN-007 binding was normalized to the total β3 integrin expression determined using a β3-integrin–specific mAb (anti-CD61–PE). This ratio, CAMTRAN-007:CD61, was then expressed as a percentage of the ratio obtained for the CHO cell clone A10 with a CAMTRAN-007 dilution of 1:50 (100%). Data are representative of 4 different experiments. Clones as per Figure 6, plus clone A06, CHO-αvβ3Leu33Arg93. - - - indicates A10; ×, A13; ●, A06;___, E05; ▴, Cam12; and ▪, Cam11.

The Arg93Gln mutation does impair the β3Leu33-specific mAb CAMTRAN-007 binding to β3 integrins expressed in CHO cells.

Adherent CHO cells were detached with EDTA buffer, washed, and directly labeled with the anti-CD61–PE or CAMTRAN-007–FITC for 30 minutes on ice. Cells were washed and analyzed by flow cytometry. To control for the variations in β3 expression between the different cell clones, CAMTRAN-007 binding was normalized to the total β3 integrin expression determined using a β3-integrin–specific mAb (anti-CD61–PE). This ratio, CAMTRAN-007:CD61, was then expressed as a percentage of the ratio obtained for the CHO cell clone A10 with a CAMTRAN-007 dilution of 1:50 (100%). Data are representative of 4 different experiments. Clones as per Figure 6, plus clone A06, CHO-αvβ3Leu33Arg93. - - - indicates A10; ×, A13; ●, A06;___, E05; ▴, Cam12; and ▪, Cam11.

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