Fig. 2.
Quantification of BFU-E in semisolid clonogenic assay.
Cells (5 × 104/mL) from marrow of patients with RA/RAEB (n = 3) or RARS (n = 3) and from healthy donors (n = 3) on day 7 of liquid culture were incubated in methylcellulose medium with Epo, SCF, IL-3, IL-6, and GM-CSF. BFU-E–derived colonies were counted after 10 days. Results are means ± SD of BFU-E colonies for 105 cells seeded. The asterisk indicatesP < .05.
