MAIPA analysis of

Gov^a andGov^b transfectants. CHO cells were transfected with Gov^a/YFP-,Gov^b/YFP-, or control YFP-expressing plasmid vectors and analyzed by MAIPA by using well-characterized Gov^a and Gov^b antisera and control blood group AB sera. Anti-Gov^a and anti-Gov^b sera react only with Gov^a- andGov^b-expressing cells, respectively. The increased signal produced with the anti-Gov^b serum reflects the known higher titer of this reagent.11 Three independent AB sera produced results similar to the AB serum data shown. CHO, untransfected cells; CHO YFP, cells transfected with the empty vector pIRES-EYFP; CHO Gov^a/YFP, cells transfected with the Gov^a-expressing pIRES-EYFP derivative pK1/YFP; CHO Gov^b/YFP cells transfected with theGov^b-expressing pIRES-EYFP derivative pK1b/YFP. Error bars indicate ± 2 SD from the mean, based on 2 experiments each performed in duplicate.