Gov genotyping by Taqman-based real-time PCR.

Samples from 21 donors in panel 1 and 2 were genotyped by Taqman real-time PCR by using conditions optimized to discriminate betweenGov^a and Gov^b alleles. Allele-specific fluorescent signals are shown. Three distinct groups, corresponding to Gov^aa (open circles), Gov^ab(open triangles), and Gov^bb (open squares), are observed. Negatives (open diamonds), no template control PCR reactions. Error bars indicate ± 2 SD from the mean.