Cytotoxicity assay of the purified CD8⁺IFN-γ⁺ and CD8⁺IFN-γ⁻ or CD4⁺IFN-γ⁺ and CD4⁺IFN-γ⁻ after sensitization with allogeneic EBV BLCL of donor B.

(A) Sensitization of donor T cells with allogeneic EBV BLCL permits the generation CD8⁺IFN-γ⁺ T cells, capable of lysing the stimulating allogeneic target cells. (B) Cytolytic activity of the CD8⁺IFN-γ⁻ cells. (C, D) No cytotoxic potential of the CD4⁺IFN-γ⁺ or CD4⁺IFN-γ⁻ subpopulations.