C/EBPε expression in bone marrow–derived macrophages.

RNA was harvested from murine wild-type and C/EBPε−/− bone marrow–derived macrophages with and without LPS (1 μg/mL) plus IFN-γγg (100 U/mL) stimulation and from human bone marrow macrophages after culture with 20 ng/mL rGM-CSF. RT-PCR–amplified products from human and mouse were blotted and hybridized using a highly conserved internal oligonucleotide probe.