Analysis of phagocytosis of

C albicans by C/EBP−/− macrophages. Macrophages from thioglycollate-elicited peritoneal lavage and bone marrow were incubated with FITC-labeled opsonized yeast. Cells were stained with trypan blue to distinguish between internalized C albicans blastopores that remained green and nonphagocytosed blastopores that merely adhered to the outer surfaces of the macrophages. (A) Cells were visualized with the use of fluorescence microscopy. (B) Phagocytosis activity of macrophages from bone marrow was calculated as the ratio of cells with fluorescence yeast to the number of total macrophages. (C) Number of internalized C albicans blastopores in each thioglycollate-elicited peritoneal lavage macrophage is shown. (▪) Wild-type macrophages; (▨) knockout macrophages. Both histograms represent the average of 3 separate experiments; each included the analysis of 2 wild-type and 2 knockout mice.